Aims of the Practical Lab Report Example | Topics and Well Written Essays - 2250 words

Aims of the Practical - Lab Report Example In the end, the recipient would have an enhanced combination of genes, as they have been donated from the donor to the recipient (Carson, Miller and Witherow, 2011, 66). Therefore, research and pharmaceuticals have an aim of quantifying the appropriate actions that should be used in strengthening a gene by fusing some genes from a recipient to a donor. The aim of this practical is to associate the transportation of genes from one form of DNA to another combination. The recombination of the DNA is basically used to ensure the recipient is at a better state than the previous state. Therefore, finding an appropriate approach towards making a better solution in strengthening gene combination and DNA is a priority in this practical. For instance, when two genes are fixed together, the bonding creates a strong combination. With such a combination, it is evident that the recipient will be at an enhanced position in the daily functionality. It is considered that fusing two genes leads to enh anced activities in the DNA, which is appropriate in keeping a highly functioning gene combination. Materials and methods There is a wide variety of materials that should be used in this process. The materials are to be readily available, to show the importance of different combinations especially in the DNA field. The initial material that will be used in this practical is the FtsZ gene. This is a single celled alga that is indicated by the Pleurochrysis carterae (Pc-ftsZ). This is a highly active protein that is believed to be the vital structure being the division of mitochondrion in the body. The protein is solely responsible for highly initiating the reaction of the mitochondria to multiply at a higher rate. Therefore, using this material in the reaction will be dominant in making an increase in the mitochondria division. The second material to be used in this experiment will be a low-copy of plasmid pProEX. The low copy plasmid will be used in comparison to the second copy of plasmid. The third material is the high copy plasmid, which will be used with the pProEX (Carson, Miller and Witherow, 2011, 31). Inserting the pProEX into the high copy of the Plasmid will be the practical of showing the reaction of the two. The other materials to be used in this experiment are gathered pBluescript KS II which is positive (+). This is a material that is gathered from E. Coli. These will be followed by a double restriction of two enzymes, namely BamHI and HindIII. The reaction will lead to a release of FtsZ, which is also vital in the experiment. After insertion of FtsZ, it will be followed by isolating pBKS II DNA, which is done by inserting a restriction enzyme. As part of the reaction, a catalyst will be used. The catalyst in this experiment will be alkaline-phosphate (AP), which is also a treatment for the lenealised plasmid. Another function of the AP will be preventing any partially digested plasmid from re-circulating. Results Plasmids are considered to be vi tal in helping bacterial hosts, in that they are useful in preventing bacterial infections. They are useful in resistance to antibiotics and degradation of organic complexes. Therefore, it is a positive maneuver for the plasmids to be increased in their production. Reproduction of the genes in the combination will obviously come out with positive results. First, the body will be well prepared to deal with organic complexes.

INVESTIGATING AUSTRALIAN RADIOGRAPHERS' PERCEPTIONS ON ADVANCED Research Paper

INVESTIGATING AUSTRALIAN RADIOGRAPHERS' PERCEPTIONS ON ADVANCED PRACTICE WITH EMPHASIS ON IMAGE INTERPRETATION AND INVOLVED TR - Research Paper Example With advanced radiographic practice, radiographers are allowed not only to acquire medical images but to perform skills formerly restricted to other specialists, such as image interpretation (Hardy and others 2008, e16) and clinical assessment. It was said that the main benefit of this system is to free physicians and other highly-trained specialists to concentrate on their essential function – that is, to diagnose and to treat (Australian Institute of Radiography 2009). United Kingdom leads the development in advanced radiographic practice (Cowling 2008), with the radiographer’s roles changing towards diagnosis and even radiation therapy over the last twenty years (Australian Institute of Radiography 2009). However, several countries still have not fully accepted advanced practice. In Australia, while there have been attempts to implement role expansion, advanced practice has not been officially established (Burrow and others 2006). This is despite the fact that the co untry has shortage of radiologists attributed to reduced working hours and small staff growth rate increase (Smith and Baird 2008). Several reasons were identified for this uncertainty in Australia including resistance from the medical workforce and radiographer education, thereby affecting radiographer’s perception towards advanced practice. Given the situation, it is necessary to explore the aspects of advanced practice in Australia since resistance to it may lead to reduced standards and recognition of the profession of the radiographers (Cowling 2008). United Kingdom vs. Australia: A Comparison The demand for radiologists due to the increasing population has been regarded as the major factor that drives the need for advanced radiographic practice worldwide. Between United Kingdom and Australia, the number of patients per radiologist is greater for the latter (Smith and Baird 2007, 629). However, when it comes to the number of examinations per year, reports show that Unite d Kingdom has more compared to Australia (Smith and Baird 2007, 629). While imbalance between supply and demand exists in these two countries, each adapted a different approach in carrying out advanced practice. According to Cowling (2008, e29), advanced radiographic practice worldwide can be classified at several levels. United Kingdom belongs to the first level which is characterized by the presence of driving forces such as government intervention, research, graduate programs and professional organizations that lead to implementation of advanced practice in the field (Cowling 2008, e29). On the other hand, Australia only belongs to the second level, in which despite the presence of the same driving forces, implementation has not been carried out to remarkable levels (Cowling 2008, e29). In United Kingdom, government policy has been directed towards enhancing the National Health Service; thus, contributing to the advancement of radiographers’ roles and practice (Australian Institute of Radiography 2009, 28). In fact, the government has increased funds for the National Health Service (Smith and Baird

Children and Nature in Poetry

Children and Nature in Poetry In numerous poems during the romantic period the themes are centered on children and nature. The themes are not just about children alone and not just nature but the two subjects together. This is to suggest that children and nature are connected, the two subjects are one. Poets during the romanticism era use the childhood period as sort of a looking glass into nature and its true aspects which seems to get lost once adulthood is reached. The poems in the romantic era are all about trying to regain that innocence that special bond that a child seems to be able to share with nature. Samuel Coleridges poem Frost at Midnight is set in the winter season with him describing his feelings about his infant baby. Coleridge realizes how special his child is and how his child is able to share a connection with nature. A connection, that Coleridge believes that he was deprived of For I was reared/ in the great city, pent mid cloisters dim, and saw naught lovely but the sky and stars(Coleridge 51-53). Coleridge here claims that because he was raised within the city he never was able to create that special bond with nature. Due to this fact he uses his child now as sort of that medium into understanding nature, since he himself lost that time of innocence to the city. Coleridge is pained by his bringing up in the city life and throughout the poem is rejoicing that his child instead will be able to grow in nature. But thou, my babe! Shalt wander like a breeze/ by lakes and sandy shores, beneath the crags/ of ancient mountain, and beneath the clouds.(Coleridge 54-56). The line Shalt wander like a breeze suggest that Coleridge believes that child and nature are one. The child will actually become a part of nature, a breeze and shall wander in nature by the lakes and sandy shores. Coleridge by the end of his poem is still hopeful for his child. That even when his child is grown, will still remember that humans and nature are one, he claims that God will help him maintain this status. Great universal Teacher! He shall mould/ thy spirit, and by giving make it ask. Therefore all seasons shall be sweet to thee.(Coleridge 63-65). Coleridge uses the child in this poem to show that children and nature are one. Through this poem the audience is meant to see that humans and nature are not separate entities but together they act as one single unit. Unfortunately as we leave childhood and begin our journey into adulthood we seem to lose that view of nature and ourselves and begin to see nature separately. Looking next at Wordsworth poem Ode Intimations of immortality from Recollections of Early Childhood Wordsworth also agrees with the Coleridge idea, that children are able to connect with nature. The only difference between the two is that Wordsworth believes that as every child is born, they immediately share a connection with nature and with heaven, that they are born with this connection. Coleridge on the other hand believes that this connection, yes, occurs during the innocent time of childhood but is not one that is guaranteed. For Coleridge this connection must be made, you are not born with it, for he himself says he never had the chance to connect with nature the way his child is able to. Wordsworth in this poem wants the audience to wake up! To see that we have lost our way and have been sleeping, he wants us to return to the mindset of a child There was a to,me when meadow, grove, and stream,/ the earth, and every common sight,/ to me did seem/ appareled in celestial light,/ the glory and the freshness of a dream.(Wordsworth 1-5). Children are the ones who are able to see nature for what is truly is. Heaven lies about is in our infancy!(Wordsworth 66). Unfortunately Wordsworth loses this way of seeing and thinking to the world as he begins to grow. It is not now as it hath been of yore; / turn wheresorer I may/ by night or day,/ the thinks which I have seen I not can see no more. (Wordsworth 5-9). Here Wordsworth is sadden that although he tries to see what he once was able to, he can not. Wordsworth believes that losing this mentality is inevitable. Our birth is but a sleep and a forgetting; Shades of the prison house begin to close/ upon the growing boy.(Wordsworth 58, 67-68). Wordsworth is sadden by this thought that we lose our innocence appreciation for the natural world. Yet he is reminded that he can still be happy for he still has the child and his memories to help connect to nature. Wordsworth uses his experiences and a child to keep him connected and Coleridge uses his infant to keep him connected. This is what it means when Child is the father of man. Of course the child can not care for the man, but still the child has something to offer. Just as a father offers protection and care for a child, the child offers the mindset and lens into the natural world. The child is a reminder that nature and humanity are one; they are intertwined with one another.

Interview with a Residential Advisor :: Interview Essay

As a residental advisor, Stephanie R worked in the academic setting in the last three years during her undergraduate years. For three years, her benefical experiences has been increased. The scope of problem she worked with the variety of problemic issues and developed a relationship with residents of the dorminity. Residents, residential advisors, Cooridators of Residental Education (CRE), and Graduate Assistants worked together in their dorm sites. The teamwork of dorms had to provide the workshops, advices, followed up with safety factors, and monitored their dorm sites. For residental advisors, their duties were largely responsible. The primary duty was safety for residents in their dorms. For example, using drug substances were major issue when it came to overdosed or allergic to them suddenly, it has to be involved with residental advisor for witness and incident report. Following up with residents after incidents were important. 　　　Before semester begins, they attended to training from two to four days. Most workshops provided the variety of topics that would prepare Residental Advisors handle the situations, residents, and personal incidents. For example, educational workshops were required of their projects. Another example, the guest speaker from non profit organization gave a presentation about domestic violences, destructive relationships, stalkers, and addictions. When residents experienced the domestic violence situations, they would make a report to residential advisors. Residential Advisors had to call their bosses to be involved. From morning to the late afternoons, they had activities and lunch breaks. They were covered by room and meal during their training days. 　　　The prevention programs were successfully approved by residents. When residents had their good relationships with their residential advisors on their floor, residential advisors provided the workshops. They came by and listened what their residental advisors wanted to educate and exchange the information with them. When their workshops ended, residents filled the survey forms and evaluated the presentation style. 　　　For example, self defensive workshop was effective and resourceful. In second year of the residential advisor professional, Stephanie and her co-worker required to present the educational workshop for residents. They in advanced asked the experienced and professional karate belt resident. He was engaged in the workshop that would help residents to know the basic self defensive tools. The main topic of workshop was how to protect yourself from harmful situations or people by using self defensive techniques. Before workshop started, residents signed the consent form that would not sue for injuries and understand the risks.

Drinking Age Controversy

In the United States, a citizen is considered an â€Å"adult† at the age of 18, and with that new title comes many responsibilities, such as the right to vote and to join the army. However, the legal drinking age in America is twenty-one. This issue has been a major controversy for some time now that faces both national and state governments. Should the drinking age be lowered to the age when legally a person becomes an adult and assumes all other adult responsibilities, or should it remain at a higher age to allow people to grow more mature and, hopefully, make more responsible decisions? In the mid 1600’s, colonial laws attempted to control alcohol consumption, but drinking per se was not remonstrated. Between 1913 and 1919 there was a lot of controversy between the â€Å"wet† states, which were states that allowed liquor, and the â€Å"dry† states, which were against and had made liquor illegal. On October 28, 1919, Congress enacted the National Prohibition Act, also known as the Volstead Act. The 18th Amendment was to become effective on January 17, 1920, and in those three months before the amendment became effective, alcohol was stolen in mass quantities from government warehouses. Throughout the thirteen years that Prohibition was enforced, the demand for alcohol only grew. The 18th Amendment was repealed on December 5, 1933 following the 21st Amendment, which was effective immediately. The National Minimum Drinking Age Act followed the 21st Amendment on July 17, 1984 stating that all states prohibit anyone under the age of 21 consuming, purchasing, or being in possession of alcohol. If the states would not comply with the law, they would not get funding under the Federal Aid Highway Act. This bill was supported by many people, one being arguably the most influential, was Candy Lightner. She founded the MADD (Mothers Against Drunk Driving) after losing her daughter in 1980 to a car accident involving a drunk driver. On average, according to MADD statistics, a person is killed by a drunk driver every forty minutes. In 2007, about 13,000 people were killed in alcohol impaired driving crashes. A â€Å"binge drinker† is defined as a person who has more than 5 drinks in one sitting, which is becoming a large issue at college campuses worldwide. Presidents from over 100 universities have voted to lower the drinking age in order to cut the amount of underage drinking on campuses. Frequent users† will drink anywhere from three times a week to everyday. A â€Å"moderate user† is someone who drinks anywhere from once a week to once a month. The decision of whether or not to keep the drinking age at 21 or lower it to 18 is continually discussed between groups such as MADD, Mothers Against Drunk Driving, who fight to keep the drinking age at 21 and LAFAA, Legalize Alcohol For All Adults, who fight to lower the drinking age. Karl Marx’s Conflict Theory is one way to explain the disagreements between those who want to lower the drinking age, and those who don’t. His theory states that social organization and change is based upon conflicts within the society. People between the ages of 18 and 21, called stakeholders, feel that they aren’t getting to exercise all of their rights to being an adult by government definition. They given all of the responsibilities and consequences of adults such as the right to vote, the ability to adopt, the right to join the military, the right to be charged with the highest punishment if accused of a crime, the right to get married, the right to buy and create pornography, etc. LAFAA would argue that with given all of these rights, government defined adults would also have to right to choose whether or not to consume alcohol. The families that allow their children to drink give a conflicting message to those who are a part of the underage drinking. Children then grow up thinking that underage drinking isn’t a big deal and is also a fun and â€Å"cool† thing to do. A large part of the â€Å"cool† factor, some would say, has to do with it being illegal to their age group. The thrill is what makes the drinking more exciting. The law also states that government usually follows majority opinion, and if this is fact, then the drinking age would be lowered seeing that two-thirds of Americans consume alcohol. Those arguing for keeping the drinking age at or above 21, such as MADD, argue that lowering the drinking age would enable younger adults to be able to purchase and drink alcohol legally, which would inevitably result in higher mortality rates. Although 18 year olds are considered adults, some think that they are still not mature enough to make to correct choices about alcohol. Those who think that keeping the drinking age at a higher minimum are trying to keep the demand for alcohol down and are also trying to keep the roads safer from drunk drivers. People between the ages of 18 and 20 will have just started college for the most part and don’t need alcohol interfering with their studies and their ability to succeed in life. Condoning the drinking of newly formed adults would only cause chaos and cause more fatalities. To MADD the cost of drinking outweighs the benefits since the highest numbers of fatalities in the United States are due to alcohol related deaths. Evidence tends to support the notion that keeping the drinking age at 21 is the safest and most responsible thing to do. Although it is taking some of Americans rights away, it is also ensuring the safety of thousands of citizens. Depending on whether you are liberal or conservative, opinions on how many rights government should take away will differ tremendously. The safety of American people should always be the most important aspect of keeping the United States a safe and enjoyable place to live. No matter whether the age is kept at 21 or lowered to 18, the controversy between the groups will never end. References http://www.druglibrary.org/Schaffer/LIBRARY/studies/nc/nc2a.htm

Analysis Vission and Mission of Coca-Cola Essay

In general, Coca-cola vision guides all members to understand what the company does, how it is done, and who it is done for also keeps the employees focused on the realistic goals of the firm, customers, investors, and other business partners. An effective vision should consists of two component which the first one is a cognitive component; which focuses on outcomes and how the best to achieve it, and the second component is affective component which will helps to motivate people and gain their commitment toward it( Boal and Hooijberg 2001). Base on Coca-cola vision statement show that they apply the affective component which increasing motivation and commitment among their employees, customers and business partners. The six P’s of Coca-cola vision which is People,, Portfolio, Partners, Planet, Profit and Productivity is the guideline to increase motivation and commitment. These six P’s also clearly mention the desirable future target for each component internally and externally of the firm, which is among people (employees), portfolio (product), partners (customers and suppliers), planet (moral obligation), profit and productivity. These visions also challenge all to participate and reach the organization goals. As a good vision, the internal (organizational beliefs and values) as well as external (shared understanding of the market environment) needs to be aligned. Besides, the Coca-cola vision statement also focuses on how the organizational members relate to the vision and shared each others. †¢People: Be a great place to work where people are inspired to be the best they can be. †¢Portfolio: Bring to the world a portfolio of quality beverage brands that anticipate and satisfy people’s desires and needs. †¢Partners: Nurture a winning network of customers and suppliers, together we create mutual, enduring value. †¢Planet: Be a responsible citizen that makes a difference by helping build and support sustainable communities. †¢Profit: Maximize long-term return to shareowners while being mindful of our overall responsibilities. †¢Productivity: Be a highly effective, lean and fast-moving organization This is a good sample of vision statement because it’s not too specific and realistic to achieve by all their members. This vision statement also encourages all members to participate in achieve the vision.